Lead Referral Laboratory
LEAD REFERRAL LABORATORY
- Lead referral laboratory (1st of its kind in Maharashtra) established on 9th Feb 2012 at Department of Biochemistry, KIMSDU, Karad, with the collaboration of National Referral Centre for Lead Projects of India, Bangalore and Indian Society of Lead Awareness and Research.
- Recognized by the WHO as a Rural Lead Referral Centre.
Details of Lead Referral Laboratory Staffs
| Name | Designation | Qualifications |
Experience (Yrs) |
| Dr. Arun J. Patil | Professor |
M.Sc. (Med) Ph.D (Med) |
27 |
|
Dr. (Mrs). Jyotsna A. Patil |
Associate Professor |
M.Sc. Ph.D (Med) |
27 |
|
Dr. (Mrs). Mandakini Kshirsagar |
Assistant Professor | M. Sc. (Med) | 10 |
| Dr. Ganesh Ghanwat | Tutor | M. Sc. (Med) | 9 |
| Mrs. Asmita Joshi | Technician | B.Sc. DMLT | 25 |
| Mr. Jaywant Thorat | Technician |
M. Sc (Med) B.Sc. DMLT |
24 |
|
Mrs. Madhura Dhaigude |
Technician |
M. Sc (Med) B.Sc. DMLT |
12 |
Details of Lead Referral Laboratory Staffs
| Name | Designation | Qualifications | Experience (Yrs) |
| Dr. Arun J. Patil | Professor |
M.Sc. (Med) Ph.D (Med) |
27 |
| Dr. (Mrs). Jyotsna A. Patil | Associate Professor |
M.Sc. Ph.D (Med) |
27 |
| Dr. (Mrs). Mandakini Kshirsagar | Assistant Professor | M. Sc. (Med) | 10 |
| Dr. Ganesh Ghanwat | Tutor | M. Sc. (Med) | 9 |
| Mrs. Asmita Joshi | Technician | B.Sc. DMLT | 25 |
| Mr. Jaywant Thorat | Technician |
M. Sc (Med) B.Sc. DMLT |
24 |
| Mrs. Madhura Dhaigude | Technician |
M. Sc (Med) B.Sc. DMLT |
12 |
- Blood lead level is measured by Lead Care II Blood lead Analyzer (ESA, Magellan Biosciences, USA).
Blood lead Samples
| Subjects | Number of Samples |
|
Battery Manufacture Workers |
907 |
|
Silver Jewellery Workers |
294 |
|
Spray Painters |
305 |
| School Children | 208 |
|
Neurological Disorder Children |
035 |
|
Healthy Adult Female |
100 |
|
Healthy Adult Male |
187 |
|
Total Blood Lead Samples |
2036 |
Research Project Completed & Published Papers
| No. | Authors Name | Title | Journal Name |
| 1 |
Shilpa A. Pratinidhi, Arun J. Patil, Manaskumar Behera, Maya Patil, Dnyaneshwari P. Ghadage, Asha K. Pratinidhi |
Effects of blood lead level on biochemical and haematological parameters in children with neurological disease of western Maharashtra, India |
J. Basic Clinical Physiology Pharmacology NovNovember 2013, Volume 25, Issue 2, Page 229-233, (PubMed, Scopus) |
| 2 |
Mrs. Mandakini Kshirsagar, Dr. Jyotsna Patil, Dr.Arun Patil, Mr. Ganesh Ghanwat, Dr. Ajit Sontakke, Dr. R. K. Ayachit |
Biochemical Effects of Lead Exposure and Toxicity on Battery Manufacturing Workers of Western Maharashtra (India): With Respect to Liver and Kidney Function Tests |
Al Ameen J. Med Sciences 2015; 8(2):107-114 |
| 3 | Mr. Ganesh Ghanwat, Dr. Arun Patil, Dr.Jyotsna Patil, Mrs. Mandakini Kshirsagar, Dr.Ajit Sontakke, Dr. R. K. Ayachit |
Biochemical Effects of Lead exposure on Oxidative Stress and Antioxidant Status of Battery Manufacturing Workers of Western Maharashtra, India. |
Journal of Basic Clinical Physiology and Pharmacology, 2016; 27(2): 141–146. (PubMed, Scopus) |
| 4 |
Mr.Ganesh Ghanwat, Dr. Arun Patil, Dr. Jyotsna Patil, Mrs. Mandakini Kshirsagar,Dr. Ajit Sontakke, Dr. R. K. Ayachit. |
Effects of Vitamin C Supplementation on Blood Lead Levels, Oxidative Stress and Antioxidant Status of Battery Manufacturing Workers of Western Maharashtra, India. |
Journal of Clinical and Diagnostic Research. April 2016, Vol.10, Issue 4, Page BC08-BC-11. (PubMed, Scopus) |
| 5 | Mrs. Mandakini Kshirsagar, Dr. Jyotsna Patil, Dr.Arun Patil, Mr. Ganesh Ghanwat, Dr. Ajit Sontakke, Dr. R. K. Ayachit. |
Biochemical Effects of Lead on Battery Manufacturing Workers of Western Maharashtra (India): With Respect to Haem Biosynthesis and Haematological Parameters. |
J Pharm Chem Biol Sci, December 2015-February 2016; 3(4):477-487. (Scopus) |
| 6 | Shilpa A Pratinidhi, Aparna A Sagare, Arun J Patil. |
Heavy Metal Levels in Commonly used Cosmetic Products in Asia. |
MIMER Medical Journal, 2018, Vol 2, Issue 2, 31-36. |
| 7 | Dr. Jyotsna A. Patil, Mrs. Mandakini S. Kshirsagar, Dr. Arun Patil. |
Activated Carbon Fabric Mask Reduces Lead Absorption and Improves the Heme Biosynthesis and Hematological Parameters of Battery Manufacturing Workers. |
Ind J Clin Biochem (2019). https://doi.org/10.1007/s12291-019-00868-3. (Scopus, PubMed) |
| 8 | Mandakini Kshirsagar, Jyotsna Patil, Arun Patil. |
Increased Blood Lead Level Alters the Heme Biosynthesis and Haematological Parameters of Spray Painters from Western Maharashtra, India. |
Toxicol. Environ. Health. Sci. Vol. 11(2), 168-174, 2019. (Scopus) |
| 9 | Mandakini S. Kshirsagar, Arun J. Patil, Jyotsna A. Patil. |
Impact of Occupational Lead Exposure on Liver and Kidney Function Tests of Silver Jewellery Workers. |
Journal of Clinical Diagnostic Research, DOI: 10.7860/JCDR/2019/38084.12442. |
| 10 | Mandakini S. Kshirsagar, Arun J. Patil, Jyotsna A. Patil. |
Biochemical Effects of Lead on Heme Biosynthesis and Hematological Parameters of Silver Jewellery Workers of Western Maharashtra, India. |
International Journal of Research Pharmaceutical Science (Accepted for Publication). |
| 11 | Mrs. Mandakini S. Kshirsagar, Dr. Jyotsna A. Patil, Dr. Arun Patil. |
Elevated Blood Lead Levels Induces Oxidative Stress and Alters the Antioxidants Status of Silver Jewellery Workers of Western Maharashtra, India. |
International Journal of Research Pharmaceutical Science, 2020, 11(4), 7254-7258. |
| 12 |
Mandakini S. Kshirsagar, Arun J. Patil, Jyotsna A. Patil. |
Increased Blood Lead Levels Induces Oxidative Stress and Alters the Antioxidants Status of Spray Painters. |
J Basic Clin Physiol Pharmacol (Accepted for publication) |
| 13 |
Mrs. Mandakini S. Kshirsagar, Dr. Arun Patil, Dr. Jyotsna A. Patil. |
Elevated Blood Lead Level Alters the Liver and Kidney Function Tests of Spray Painters. |
Indian Journal of Forensic Medicine and Toxicology. (Accepted for Publication |
| 14 |
Mrs. Mandakini S. Kshirsagar, Dr. (Mrs). Jyotsna A. Patil, Dr. Arun J. Patil. |
Effects of Supplementation of Multivitamins and Multiminerals on Blood Lead Level, Oxidative Stress and Antioxidant Parameters of Silver Jewellery Workers from Western Maharashtra, India |
Australian Journal of Occupational Therapy (Submitted on 6.3.2020) |
| 15 |
Mrs. Mandakini S. Kshirsagar, Dr. (Mrs). Jyotsna A. Patil, Dr. Arun J. Patil. |
Elevated Blood Lead Level Alters the Heme Biosynthesis and Haematological Parameters of Unorganised Batter Manufacturing Workers from Western Maharashtra, India. |
Journal of Environmental Pathology, Toxicology and Oncology (6.3.20200 |
| 16 |
Dr. Mandakini S. Kshirsagar, Dr. Jyotsna A. Patil, Dr. Arun J. Patil. |
Alteration of lipid profile in lead exposed individuals from Western Maharashtra |
International Journal of Pharmaceutical Research | Jul- Sep 2020 | Vol 12 | Issue 3. 2807-2811. |
| 17 |
Dr. Jyotsna A. Patil, Dr. Mandakini S. Kshirsagar, Dr. Arun J. Patil. |
Daily Use of Activated Carbon Fabric Mask Decreases the Blood Lead Levels, Oxidative Stress and Improves the Antioxidant Status and Liver Functions of Unorganized Battery Manufacturing Workers |
International Journal of Research Pharmaceutical Sciences, 2020, 11(4), 7247-7253. |
| 18 |
Mandakini S Kshirsagar, Jyotsna A Patil, Arun J Patil. |
Elevated blood Lead impairs the Hematological and Heme Biosynthesis related parameters of Silver Jewellery Workers. |
Int. J. Res. Pharm. Sci., 2021, 12(1), 662-668. |
Completed Projects - Lead Referral Laboratory
| Sr. No. | Name of Projects |
| 1 |
Estimation of blood lead level and its biochemical effects on battery manufacturing workers before and after using mask. Dr. Jyotsna Patil |
| 2 |
Study of Relevant Biochemical Parameters in Lead Exposed Individuals of Western Maharashtra Mr. Mandakini Kshirsagar (Ph.D Research) Awarded |
| 3 |
Study of Blood Lead Levels in Antenatal Woman and Fetal Outcome- Dr. Shilpa Pratinidhi |
| 4 |
Biochemical effects of blood lead level on children with neurological disorders of Pune (Maharashtra), India. Dr. S. A. Pratinidhi |
| 5 |
Biochemical Effects of Lead Exposure and Toxicity on Battery Manufacture Workers of Western Maharashtra (India): With Respect to Oxidative Stress and Antioxidants Status- Mr. Ganesh Ghanwat, |
| 6 |
Biochemical Effects of Lead Exposure and Toxicity on Battery Manufacture Workers of Western Maharashtra (India): With Respect to Heme Biosynthesis, Liver and Kidney Functions tests. Mrs. Mandakini Kshirsagar, |
| 7 |
Estimation of Lead level from water samples of Koyna and Krishna river beds. (Forty water samples collected from the tube well / wells which are situated about 80ft to 100ft away from both the sides of the river at different spots- Dr. Arun Patil |
| 8 |
Lead estimation from soils of different spots to have a scientific data of the existing condition of the water along the Koyna and Krishna river beds Dr. Arun Patil |
| 9 |
Estimation of lead content from of standard and local made water pipes- Dr. Arun Patil |
| 10 | Estimation of Blood lead levels of school Children's of Karad - Dr. Arun Patil |
Ongoing Projects - Lead Referral Laboratory
| Sr. No. | Name of Projects |
| 1 |
Biochemical Effects of Lead on Steel Welders- Dr. Arun Patil |
| 2 |
To conduct extramural, multicentre and collaborative research project on topic “Biochemical Aspects of Lead Exposure on Traffic Police at Some District Places of Maharashtra and Karnataka (India).- Dr. Arun Patil |
| 3 |
Effect of serum lead on salivary Visfatin levels in Periodontitis patients using smoke or smokeless form of tobacco. Dr. Harshada Zagde |
| 4 |
Study of Nickel, Chromium, Cobalt leaching from dental casting alloys and their effects on human health. Dr. Shubha Joshi |
| 5 |
Relation between blood lead levels and childhood anaemia. Dr. Ankit Gupta |
| 6 |
Impact of Chelating Agent Therapy, with and without Supplementation of Antioxidants on the Biochemical and Haematological Parameters among the Lead Intoxicated Workers- Ms. Sophiya Waidande |
List of Ph.D Students
| Sr. No. | Thesis Title |
| 1 |
Study of Relevent Biochemical Parameters In Lead Exposed Individuals of Western Maharashtra – Dr. MRS. MANDAKINI S.KSHIRSAGAR (Awarded) |
| 2 |
Impact of Chelating Agent Therapy, with and without Supplementation of Antioxidants on the Biochemical and Haematological Parameters among the Lead Intoxicated Workers- Ms. Sophiya Waidande (Ongoing) |
| 3 |
To Study the Effects of Lead Exposure on Silver Jewellery Workers and Their Family Members Before and After the Supplementation of Vitamin E, Installation of Chimney and Use of Activated Carbon Fabric Mask at Their Workplace with Respect to Biochemical and Haematological Parameters. Ms. Aneesa Sayed (Registered) |
| 4 |
Study of Nickel, Chromium, Cobalt leaching from dental casting alloys and their effects on human health. Dr. Shubha Joshi |
Proposed Plan of Activities
| Sr. No. | Activities |
| 1 |
Screening programme for blood lead level of the school children and occupationally lead exposed population such as battery manufacturing workers, silver jewellery workers, spray painters, traffic police, petrol pump workers. of the Western Maharashtra (India). |
Extramural International Grants
- The Underwriters Laboratories (UL) USA has sanctioned Rs. 25 lakhs grant to the InSLAR) Lucknow, for assessing water and blood lead levels in five cities of India. Out of which 5 lakhs are sanctioned for KIMSDU LRL and 2 lakhs are released.
- Sanctioned 1 lakh by NRCLPI (WHO grants) for estimation of blood lead level of 120 school children.
Educational & Awareness
- Prepared the posters & power point presentation about Lead exposure and toxicity for creating lead awareness among the school children and lead exposed populations.
- Informed the all battery manufacturing industries of Maharashtra regarding the lead exposure and toxicity and facility available for blood lead estimation in KIMSDU, Karad.
Awards
- Lead Referral Laboratory, KIMSDU, Karad has been awarded the outstanding Best Performance Award for the year 2013 by National Referral Centre for Lead Projects in India (NRCLPI) among the 36 Lead Referral Laboratories all over India.
- Successful Establishment of Lead Referral Centre in 1st International Conference on Lead at AIMS, Jodhpur on Oct. 2015
Awards
- Presented papers and Received Three Prizes in LEADCON 2018 “4th International Conference on Lead organized by Medical Education Unit, North Bengal Medical College and Hospital, Siliguri.
1. Dr. Jyotsna Patil, presented paper on “Effects of Lead on
Oxidative Stress and Antioxidants of Spray Painters,
Western Maharashtra, India” and received third Position
Award.
2. Dr Shubha Joshi, presented paper on “Base Metal Alloys
used in Dental Restorations” and received the Second
Position Award.
3.Dr Shilpa A. Pratinidhi, Dr Arun J. Patil presented paper
on “Heavy Metal Levels in Commonly Used Cosmetic
Products in Asia and received First Position Award.
Awards
- Biochemistry staff presented the following three posters in LEADCON 2016 and bagged the second prize.The prize money was equally divided and paid to three posters as shown below.
1.Effects of Lead on Haem Biosynthesis and Haematological Parameters in Battery Manufacturing Workers of Western Maharashtra, India - Mandakini Kshirsagar
2.Biochemical effects of lead exposure and toxicity on battery manufacturing workers of Western Maharashtra (India): with respect to liver and kidney function tests.-Dr. Jyotsna Patil
3.Effect of Vitamin C Supplementation on Blood Lead Level, Oxidative Stress and Antioxidant Status of Battery Manufacturing Workers of Western Maharashtra, India. Mr. Ganesh Ghanwat
LEADCON Participation
| Sr. No. | Participants | Resposibility | Conference Name |
| 1 |
Dr. Arun Patil |
Panelist member, Resource & Chair Person |
1st International Conference on Lead at AIMS, Jodhpur on Oct. 2015 |
| 2 |
Dr. Arun Patil Dr. Jyotsna Patil |
Panelist member, Resource & Chair Person Presented Paper |
3rd LEADCON 2017, at NIN, Hyderabad on Sept . 2017 |
| 3 |
Hon’ble Dr. Vedh Prakash Mishra Dr. Arun Patil Dr. Jyotsna Patil Dr. Shilpa Pratinidhi Dr. Shubha Joshi |
Guest of Honor Resource & Chair Person Guest of Honor, Resource & Chair Person Resource & Chair Person Resource & Chair Person Resource & Chair Person |
4th LEADCON 2018, at North Bengal Medical College and Hospital, at Siliguri. |
| 4 |
Dr. Arun Patil Dr. Jyotsna Patil Dr. Shubha Joshi |
Panelist member, Resource & Chair Person Resource Person Resource Person |
LEADCON 2020- at NIMHANS, Bangalore on 9th & 10th Feb 2020 |